Contact an Account ManagerRapid method based on reversed phase HPLC for purification of human myelin basic protein and its thrombic and endoproteinase Lys-C peptides
Summary
Type: HPLC
Application #: 108050
Catalog#: 720059.40 - MN HPLC Column, Nucleosil C4, 300A, 5um, 4x250mm
Detection: UV, 210nm
Conditions: eluent A: 0.1% trifluoroacetic acid (TFA) in water, eluent B: 0.09% TFA in acetonitrile, 5min 10% acetonitrile, gradient from 10 to 35% acetonitrile in 50min, from 35 to 80% acetonitrile in 10min, from 80 to 10% acetonitrile in 5min
flow rate 1ml/min
Authors: Giegerich G
Source: J Chromatogr 528 (1990) 79 - 90
Details
Legend: Fig. 7. Separation of Lys-C-produced peptides of hMBP fragment 1-97.